DOI: 10.5176/2251-2489_BioTech13.85

Authors: Susmita Shukla and S.K. Shukla

Abstract:

Dioscorea hispida Dennst is the important member of family – Dioscoreaceae and commonly known baichandi. In India, people consume sweet baichandi chips of the processed tubers contains high contents of carbohydrates. They can be vegetatively propagated through tubers, which is a cumbersome and slow process. Therefore micropropagation technique was applied for the mass multiplication of this species. In the present study attempts were made to evaluate the effect of adjuvants on in vitro shoot proliferation of Dioscorea hispida. The nodal segments of Dioscorea hispida was established in WPM medium supplemented with 1.0 mg/l BAP. Various additives were added in the medium to increase shoot multiplication in D. hispida. The highest number of shoot proliferation was obtained when nodes of Dioscorea hispida were placed on WPM supplemented with 1.0 mg/l BAP and 1000.0 mg/l PVP. Besides, it controlled the blackening/ browning of the medium. Maximum root induction were obtained when the in vitro grown shoots of D. hispida placed on half - WPM with 2.0 mg/l IAA. Rooted plantlets were acclimatized with 90 % survival rate.

Keywords: Dioscoreaceae, Micro propagation,WPM, PVP, Browning

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