DOI: 10.5176/2251-2489_BioTech16.16

Authors: Ismail Soner KOLTAS, Ramazan BILGIN

Abstract: Fasciola hepatica, also known as the common liver fluke or sheep liver fluke, is a parasitic trematode of the class Trematoda, phylum Platyhelminthes that infects the livers of various mammals, including humans. The disease caused by the fluke is called fascioliasis, which is a type of helminthiasis and has been classified as a neglected tropical disease. Catalase (EC 1.11.1.6) present in the peroxisomes nearly all aerobic cells, serves to protect the cell from the toxic effects of hydrogen peroxide by catalysing its decomposition into molecular oxygen and water without the production of free radicals. In the present study, we aimed to investigate the role of antioxidant enzymes against toxic reactive oxygen species in human fascioliasis. This study was conducted on patients with fascioliasis and compared with healthy controls. Fasciola hepatica was determined by microscopy and antibody detection (Indirect haemagglutination test) analysis. We assayed catalase levels measured of 24 subjects and matched in 36 healthy controls. The catalase activity was measured by Luck. The difference between catalase levels of patients (13,86x104±2,07x104 IU/mg hemoglobin) was lower than control groups (17,23x104±1,87x104 IU/mg hemoglobin) and was statistically significant (p<0.05). In conclusion, there is an increase in oxidative stress in a fascioliasis. Despite this stress, the antioxidant system is deficient and adequate, in patients with fascioliasis.
Keywords: Fascioliasis, flukes, Indirect haemagglutination test

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