Authors: Nisansala S. Bopage, Prof G. M. Kamal B. Gunaherath, Senior Lecture K. Hector Jayawardana, Prof Chandrani Wijeyaratne, Ajita M. Abeysekera
“Seetodaka” oil is a medicinal oil which is been used in the treatment and management of fresh wounds by indigenous medicinal practitioners in Sri Lanka. The oil is prepared by boiling a mixture of aqueous extracts of, rhizome of Curcuma longa, leaves of Azadirachta indica and roots of Asparagus racemosus with coconut milk. The wound healing activity of the oil and these plant materials was studied using in-vitro Scratch Wound Assay (SWA) on Baby Hamster Kidney (BHK 21) and Madin-Darby Canine Kidney (MDCK) cell lines. Hexanes fraction of “Seetodaka” oil and the hexanes fraction of A. racemosus and the EtOAc fraction of C. longa were found to be responsible for the enhancement of wound healing on both BHK and MDCK cell lines. The activities of hexanes fraction of A. racemosus and EtOAc fraction of C. longa were found to be concentration dependent and exhibit an optimum values of 12.5 mg dm-3and 75 mg dm-3for respectively on both cell lines. β-Sitosterol was found to be one of the major compound present in both hexanes fractions of “Seetodaka” oil and A. racemosus and it has shown strong wound healing activity over SWA comparable to the positive control, asiaticoside. Antibacterial assay using Kirby-Bauer disc diffusion method against Bacillus sp. and E. coli revealed that all fractions of A. indica are moderately active.
Keywords: Seetodaka” oil; Scratch Wound Assay (SWA); Baby Hamster Kidney (BHK 21)cells; Madin-Darby Canine Kidney (MDCK) cells; Curcuma longa; Azadirachta indica; Asparagus racemosus; β-sitosterol;