Authors: Jebunnesa Chowdhury and Aparna Islam
Abstract: The present study was performed to formulate a reproducible and standardized in-vitro regeneration system for most popular and commercially important tomato varieties of Bangladesh. As establishment of tissue culture protocol is a prerequisite for transformation experiments, the present endeavor were aimed to develop a reliable and reproducible in vitro regeneration protocol for three of our locally grown popular tomato varieties, namely, Bahar (BR), Bina tomato 5 (B-5), Bina tomato 3 (B-3). In addition to that the established protocol was compared with one of the most popular Indian commercial variety, Pusa Ruby (PR). For initiation of the culture, cotyledonary leaf explants were collected from 8-10 days old tomato seedlings. To promote germination, agitation of seeds following sterilization was found effective. MS media containing 2 mg/l 6-benzylaminopurin (BAP) showed best shoot regeneration with maximum number of shoots for all four varieties and response was more than 80% in all the varieties. Rooting at the base of the regenerated shoots was best in half strength MS media supplemented with 0.2 mg/l indole-3 acetic acid (IAA). The regenerated plantlets successfully acclimatized in soil, where they flowered and formed fruits. Seeds collected from these fruits were found to be viable in germination tests. During these comparative studies, the present protocol showed similar response in both local and imported tomato varieties and proved that established protocol is applicable to wide range of genotype.
Keywords: Regeneration frequency, Comparative study, Cotyledonary leaf explant, popular tomato