Authors: Musa M. Musa
Secondary alcohol dehydrogenase from Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase (TeSADH) is of considerable interest as a biocatalyst. The effect of the structure of substrate on stereoselectivity of TeSADH-catalyzed reduction reactions has been studied. The results demonstrate that a small change in the substrate can cause a significant change in the stereoselectivity of TeSADH-catalyzed reactions. The asymmetric reductions of 1- phenyl-2-propanone, 1-phenyl-2-butanone, and 1-phenyl-2- pentanone have been studied. The results revealed that there is a significant improvement in the enantioselectivity of the TeSADHcatalyzed reduction of 1-phenyl-2-butanone in comparison with that for 1-phenyl-2-propanone and 1-phenyl-2-pentanone. The study was conducted using W110A TeSADH mutant.
Keywords: Alcohol dehydrogenase, asymmetric reduction, biotransformations, Prelog’s rule