DOI: 10.5176/2251-2489_BICB11
Authors: Seyyed Javid Mortazavi Tabrizi, Aliakbar Barmaki and Hamid Mirzaii
Abstract: Lactic Acid Bacteria (LAB) are constituted of a heterogeneous group of Gram-positive bacteria with a strictly fermentative metabolism from which lactic acid is the key metabolite. During this research about 40 cat fish (Silurus glanis) hunted from Aras River in East Azarbaijan, Iran, randomly. Then samples under sterile condition and nearice transferred to veterinary faculty microbiology laboratory, and after dissection, about 1 gram fecal from fore-gut were sampling and pour into diluting solution then about some minute mixed, then dilution serial provided for counting lactobacillus and 0.1 ml from each dilution, cultured in surface of MRS agar. For provide best growth condition oflactobacillus, incubated them in anaerobic jar with gas pack type C about 48-72 hours in 37oc. We extracted DNA bacteria after phonotypical and biochemical identity of samples, then PCR experiments accomplished by use of special primers for distinction of lacto bacillus species. And in order to final distinction, PCR products after enzymatic digestion of samples, transferred to Faza Pajhouh Company for found sequencing. from 40 samples cat fish from Aras river, only 2 cases of samples were positive for lacto bacillus and following biochemical experiments, species of the two lactobacillus identified as lactobacillus plantarum and in each gram of positive fecal samples about 3.75103 CFU/g and 1.75102 CFU/g of lactobacillus plantarum counted and conformed respectively. Results of 16s rDNA sequencing that had produced by forward and reverse primer by use of BLAST (bl2seq) and chromas bioanformatic software analyzed and aligned. Results of sequencing about isolated bacteria indicated that this species has been lactobacillus plantarum and the founding’s corresponding to produce results from biochemical tests and determine cutting pattern for this species.
Keywords: cat fish, Aras River, PCR
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