DOI: 10.5176/2382-5685_VETSCI14.16
Authors: AMNA AZAD, ZAINAB WAHAB and FARHANA RIAZ CHAUDHRY
Abstract:
Anthelmintics are central to the treatment of trematodiasis. But this orthodox have been threatened due to development of resistance. The upcoming surrogates are the medicinal plants which contains complex chemicals called secondary metabolites that are responsible for self-defense against pathogens. An adult mortality assay involving Paramphistomum cervi and Fasciola gigantica was used to evaluate the flukicidal activity of methanol extracts from four medicinal plants that are native of of Gilgit-Baltistan-Pakistan. Across the whole series of tests carried out, methanolic extract concentrations of Zanthoxylum alatum (p<0.05), time of exposure (p<0.05) and their interactions (p<0.05) had statistically significant effects on the number of dead flukes (Paramphistomum cervi) as compared to Punica granatum, Juglans regia and Berberris lycium, respectively. While similar series of significant results against Fasciola gigantica were monitered by the Punica granatum followed by B. lycium, Z. alatum and J regia, respectively. Both plants not only show high degree of trematocidal activity but they can produce significant damage within fluke tegument. This revealed through our histological studies that P. garanatum and Z. alatum cause severe erosion on fluke tegument as compared to positive control (Albendazole). These results support the need for invivo evaluations of the flukicidal properties for at least these two plants.
Keywords: Adult motility assay, Flukicidal potential, Medicinal plants, Histology, Tegument
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