DOI: 10.5176/2382-5685_VETSCI14.29
Authors: Mohamed Nayel, Akram Salama, Ahmed Elsify, Mahmoud allaam, Salah EL –Ballal, Ibrahim Eldesoky, Doaa Khalaf, Mohamed Effat, and Mona Al Shabrawy
Abstract:
This study focused on determining the frequency and morphological identification of yeast involved in unresponsive calves’ diarrhea by antibiotics, with molecular identification of C. albicans. C. albicans the most frequently isolated yeast in this study and its Phospholipase B1 (PLB1) gene as a virulence factor. One hundred fecal samples from diarrheic calves were investigated, 49 strains of yeast were isolated. Thirty four different strains (69.3{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) classified as Candida were identified based on phenotypic properties. The dominating species were C. albicans (20.4{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) Followed by C. glabrata (16.3{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), G. candidum (14.3{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), C. krusei (12.2{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), C. tropicalis (10.2{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), C. guilliermondii (10.2{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), R. rubra (8.2{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) and C. neoformans (8.2{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}). seven out of ten strains phenotypically identified as C. albicans were confirmed by PCR using specific primers for the amplification of a fragment of the rRNA gene of C. albicans. Phospholipase B1 gene a virulence determinant of C. albicans, was detected in all molecularly identified C. albicans isolates. The results of the present study indicate the importance of PCR as more rapid, effective and reliable tool for screening of yeast spp. than the phenotypic methods. When confronted with cases of undifferentiated calf diarrhea to avoid unnecessary and potentially harmful antibacterial therapy.
Keywords: Diarrhea, Yeasts, C. albicans, Calves, PCR
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