DOI: 10.5176/2251-2489_BioTech15.07
Authors: Rutuja G. Vaze, Annamma Odaneth and Arvind M Lali
Abstract: Protein hydrolysates from natural sources have been reported for bacterial growth as they contain growth promoting bioactive peptides. In current work, peptides are generated by hydrolysis of non animal proteins such as soy proteins; these non animal proteins overcome drawbacks of animal proteins (virus contamination, cost, undefined composition) which are conventionally used as nitrogen source for cell proliferation. Molecular weight of peptides plays important role in cellular metabolism; thus peptides differing in molecular weights are generated using enzymatic hydrolysis as proteolysis could be restricted by controlling protease catalyzed reaction. Differential hydrolysis is carried out by use of immobilized alkaline protease; controlling the enzyme and substrate contact time. The effect of degree of hydrolysis on cellular proliferation is being tested. Suitable hydrolysis conditions are being defined in order to produce protein hydrolysate which aid in improvement of cell growth. This study will compare influence of protein source and molecular weight distribution of peptide preparation i.e. hydrolysates on their ability to be used for enhanced growth of bacterial and animal cells.
Keywords: bioactive peptides, cell proliferation, bacteria, animal cell culture
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