DOI: 10.5176/2251-3833_GHC16.49
Authors:Nuraniza Azahari, Muhammad Muzaffar Ali Khan Khattak, Nor Azwani Mohd Shukri
Abstract: The objective of this study was to isolate, quantify total protein & adiponectin from trimmed off abdominal adipose tissues from halal meat sources namely chicken, beef and lamb. Abdominal adipose tissues were isolated from the aforementioned sources and rinsed with ice-cold phosphate-buffered saline (PBS) solution. Delipidation of the tissues were performed by chloroform/methanol extractions. The protein concentration was determined by using Protein Assay Bicinchoninate Kit method. This was followed by quantification of the adiponectin using ELISA kit assay. The experiment was conducted in triplicates and the results were presented as means ± SD. Results indicated that the extraction of 10 gram subcutaneous adipose tissues from chicken, beef and lamb yielded 0.10 gram of protein amount which was 1 {6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} from the total tissue mass. The protein concentration in adipose tissue of chicken, beef and lamb were 1.25 ± 0.05, 1.75 ± 0.05 & 2.53 ± 0.07 mg/ml respectively. The adiponectin concentration in chicken, beef and lamb were 158 ± 0.05 ng/ml, 24 ± 0.05 μg/ml and 37 ± 0.08 ng/ml respectively. Adiponectin from these sources may be one of the promising candidates for future pharmacological treatment strategies for insulin resistance and metabolic diseases.
Keywords: Adipose tissue; Protein; Adiponectin; BCA kit; ELISA Assay
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