DOI: 10.5176/978-981-08-8119-1_Biotech19

Authors: Nor-Zuhailah, M. Fatihah, N.H.N., Nashriyah, M, Ali and Choong Chee Yen

Abstract: Internal transcribed spacer (ITS) was used as the molecular markers to determine the genetic relationship of Ficus deltoidea. The strict consensus tree determined that six varieties of Ficus deltoidea was clustered in subsection Frustescentiae with 82{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} bootstrap support. Ficus ischnopoda was the sister to this clade followed by Ficus erecta. Variety intermedia was the first variety evolved among Ficus deltoidea varieties. However, the clade of Ficus deltoidea was paraphyletic because Ficus oleifolia was ingroup with variety deltoidea with 68{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} bootstrap support. Variety bilobata was clustered into two different clades. Variety bilobata (FD14) was genetically closer to variety trengganuensis and variety kunstleri whilst and variety bilobata (FD13) was closer to variety angustifolia. The molecular systematics result from ITS data alone does agreed that classification of Ficus deltoidea complex in section Frutescentiae but do not support the latest classification by Berg (2003) and Berg and Corner (2005) that classified variety intermedia in subspecies Ficus oleifolia

Keywords: Ficus deltoidea, rDNA and ITS

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