DOI: 10.5176/2251-3140_2.1.17
Authors: David J Bissonnette, M Hadley, Ying He, Stan Kubow
Abstract:
It was been hypothesized that critical illness will change the activity of phospho-fructokinase (PFK) and that intramuscular (i.m) injections of the antioxidant α-tocopherol would protect this ratelimiting enzyme of glycolysis in the slow-twitch soleus and in the fast-twitch extensor digitorum longus (EDL) muscles of metabolically-stressed and malnourished rats. Twenty eight male Wistar rats were divided into four groups: controls (CNs), zymosan-injected (ZY), α-tocopherol-injected (ZYE), and pair-fed (PF). Rats were fed Osmolite-HN, and were sacrificed on day three. PFK
activity was measured in EDL and soleus muscles using a fluorometric assay, and was found to be significantly lower in both the soleus and EDL of ZY rats. The PFK activity in the EDL of ZYE rats was comparable to the PFK of PF rats and was significantly higher than ZY rats on day three. Glycolysis in the slow- and fast-twitch muscle of ZY rats is adversely affected, and three days of i.m injections of α-tocopherol significantly (p<0.05) protected PFK activity, in fast-twitch but not in slow twitch muscles.
Keywords: Critical illness, Fast-Twitch Muscles, Glycolysis, Phosphofructokinase, Slow–twitch muscle, Antioxidants, Trauma.
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