DOI: 10.5176/2251-2489_BioTech13.64
Authors: Waldemar Rymowicz, Agnieszka Kita, Anita Rywińska, Aleksandra Mirończuk
Abstract:
The ability of yeast strains to assimilate glycerol derived from biodiesel to produce an important metabolic compounds for the food industry, such as erythritol, was verified. Glycerol was used as a substrate for the erythritol production by a new isolated mutant of Yarrowia lipolytica MK1, in fed-batch fermentations. An effect of various amount of ammonium sulfate as a nitrogen source and an initial glycerol concentration on the production of erythritol by MK1 strain during fed-batch fermentations was investigated. During fed-batch fermentation under optimal experimental conditions, 300 g/L of glycerol was converted into erythritol, achieving a final erythritol concentration of 155 g/L, a conversion yield of 0.52 g/g and a volumetric productivity of 1.1 g/Lh. Biomass of Y. lipolytica as a by-product of this process contains a suitable amount of essential amino acid and unsaturated fatty acid. Therefore, it might be used as a good protein and lipids sources for animal feed. In summary, the current study confirmed the utilization of glycerol is an appropriate substrate for the production of erythritol by newly isolated yeast strains under different experimental conditions.
Updating...