DOI: 10.5176/2251-2489_BioTech
Authors: Md. Hasanuzzaman and Idris A. B.
Abstract: The carambola fruit fly, Bactrocera carambolae Drew and Hancock (Diptera: Tephritidae) is a major agricultural pest in Malaysia which causes serious economical damage in the fleshy fruits and vegetables. The electrophoretic banding patterns of nonspecific esterase isozymes were observed for the first time on vertical Polyacrylamide Gel Electrophoresis (PAGE) during the different life stages of B. carambolae. Three esterase isozymes, EST-1, EST-2 and EST-3 were observed and their relative mobility values were measured. EST-10.61 was highest mobility and close to the anode. EST-30.15 was lowest mobility and close to the cathode. EST-10.61 and EST-20.46 were present in larvae; and EST-20.46 and EST-30.15 were observed in adults; whereas EST-30.15 was found in pupae. The activity of EST-30.15 was highest in case of adult flies compare to pupae and EST-20.46 showed highest activity in adults than larvae. There was no activity in eggs. Esterase isozymes are involved in the various physiological processes of insects. Data presented here will be helpful for developing environment friendly novel control methods of this pest and also for comparison to other Bactrocera species.
Keywords: electrophoresis; carambola fruit fly; ontogeny; polyacrylamide gel; tephritidae
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