DOI: 10.5176/2382-5685_VETSCI13.76
Authors: Shadia Sharmin, Begum Safia Zaman, Md. Ariful Islam and Mst. Minara Khatun
Abstract:
The study was conducted to evaluate bacterial flora of salad vegetables and for isolation, identification, characterization and antibiogram studies of the organisms obtained. For this, a total of 90 samples from mixed vegetables which are commonly used for salad such as tomato, lemon, green chili, coriander leaf, carrot and cucumber were collected from five different markets located in Mymensingh city. All the vegetables were highly contaminated with bacterial flora. Range of microbial count of tomato was log 6.276 CFU/ml to 6.543 CFU/ml, lemon was log 5.493 to 6.261 CFU/ml, green chili was log 5.205 to 5.64 CFU/ml, coriander leaf was log 7.055 to 7.759 CFU/ml, carrot was log 6.786 to 7.221 CFU/ml and cucumber was log 5.469 to 6.845 CFU/ml, respectively. All these samples were also analyzed by culturing in different selective media such as Salmonella-Shigella (SS) agar, Eosin Methylene Blue (EMB) agar, Acetamide agar, M- Aeromonas (MA) agar, Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar and Nutrient agar (NA). Biochemical tests were performed to identify the organisms. Out of 90 salad vegetable samples a total of 234 bacterial isolates of six genera were identified. Pseudomonas spp. (28.20{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) was the most dominant followed by Bacillus spp. (20.94{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), Aeromonas spp. (20.51{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), Vibrio spp. (16.66{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), Salmonella spp. (8.11{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) and E. coli (5.55{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), respectively. Results of antibiotic sensitivity test showed that, out of five antibiotics Salmonella sp., Bacillus sp., Pseudomonas sp., Aeromonas sp. and E. coli were equally sensitive to three antibiotics such as Chloramphenicol, Ciprofloxacin and Gentamicin and resistant to Ampicillin and Cefalexin, respectively. Whereas Vibrio sp. was sensitive to Chloramphenicol and Gentamicin, intermediately sensitive to Ciprofloxacin and Ampicillin and resistant to Cefalexin. In this study the bacterial load was highest at Natunbazar and lowest bacterial load was recorded in KR market at BAU campus. These results suggest that the necessity to follow the hygienic practices of handling the salad vegetables in local markets of Mymensingh city.
Keywords: Salad vegetables, Microbiological quality, Multi-drug resistant bacteria, Mymensingh city
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