DOI: 10.5176/2251-2489_ BioTech14.01
Authors: Kirti Rani
Abstract: Albumin easily biodegrades into natural products (amino acids) and non-toxic as well. Due to these exploitable features, it is excellent bio-matrix material for the immobilization/encapsulation/ entrapment. Amylase is a well known industrial important enzyme which digests starch into maltose from the non-reducing end and thus produces, limit dextrins. This property of amylase has a wide application in food, pharmaceutical, paper, leather, detergent and textile industries. Albumin is main constituent of total protein in human plasma and its biodegradation takes place into natural products such as amino acids which is non-toxic nature and nonantigenicity too. Thus due to these exploitable features of albumin, it is easily metabolized in the body. Hence, in present study, egg albumin was used as biocompatible matrix for entrapment of enzyme. And, entrapment of Glycine max amylase was done into chemically modified egg albumin whose chemical modification was done by toluene. Various kinetic properties were studied for Glycine max amylase and its {6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} of retention of enzyme activity was estimated after the chemically modified entrapment into egg albumin. Biodegradation of entrapped Glycine max amylase was done by using alkaline protease to study its controlled and consistent release from chemically modified egg albumin. This chemically modified entrapment of enzyme in to egg albumin was led to enhanced stability of enzyme on preservation as compared to free enzyme.
Keywords: Glycine max; egg albumin; glutaraldehyde; toluene
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