DOI: 10.5176/2251-2489_ BioTech14.11
Authors: Prakash Sarwa and Sanjay Kumar Verma
Abstract: Wastewater treatment using biosorption technology is a major area of research worldwide in recent years due to its efficiency and eco friendly nature. The aim of the present study was to evaluate the quality of Zn (II) contaminated water treated with microalga, Scenedesmus sp. MCC 26 in Swiss albino mice. Assessment of toxicity of algae treated Zn (II) solution was done in mice by investigating its hematological, biochemical variables and histopathology of liver and kidney. The Mice were allocated evenly into three groups: Group I: Control group, Group II (Untreated Zn group): Zinc sulphate solution @11.5 mg/kg body weight (bw) was administrated intraperitoneally (i.p ) to mice on alternate day for a period of 28 days. Group III (Algae treated Zn group): Zinc sulphate solution treated with (0.3g dry weight) algae for 5hrs and resulting filtered solution was administrated i.p to mice on alternate day for duration of 28days. A comparison of hematological parameters in mice indicate that level of white blood cells (WBC) and Platelet counts (PLT) significantly increased in untreated Zn group compared to control. In addition, the count of red blood cells (RBC) and Hemoglobin (Hgb) showed a decline in untreated Zn group over control. However, algae treated Zn group, showed insignificant increase in WBC and PLT and no decline in RBC and Hgb over to control. Liver and kidney markers such as lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST) alkaline phosphatase (ALP) urea nitrogen (BUN) and creatinine (CR) showed significant enhancement (p<0.05) in untreated Zn group while no such significant increase in biochemical parameters were observed in algal treated Zn group compared to control. Histological results showed some alterations in normal architecture of liver and kidney in untreated Zn group but it was observed that algal treatment of Zn water prevented the Zn (II) induced alterations in both organs of mice.
Keywords: Zinc; Swiss albino mice; Microalgae; Bisorption; Liver; Kidney
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