DOI: 10.5176/2382-607X_ARP14.04
Authors: Lingling Zhong, Ping Zhou
Abstract: The present study explored the effects of H2S on human platelet in vitro and on mouse platelet ex vivo, which was also studied under dyslipidemia conditions. NaHS at concentrations from 0.01 to 10 mM inhibited the aggregation of washed human platelets with an IC of 93.8 μM. The collagen-induced platelet aggregation, ATP release, and TXA2 formation were also inhibited by NaHS incubation. Furthermore, NaHS significantly decreased intracellular calcium level in washed human platelets stimulated with collagen and inhibited collagen-induced c-PLA2, p38 MAPK, ERK, JNK, PLC- γ2 and Akt phosphorylation. Injection of NaHS (1 μmol/g ) into mice significantly inhibited the collageninduced platelet aggregation. Finally, NaHS inhibited the aggregation of washed human platelets induced by ox-LDL plus collagen. The collagen-induced rapid platelet aggregation in apoE knockout mice was also significantly decreased by NaHS treatment. Our study showed that H2S was able to inhibit platelet aggregation induced by collagen. The underlying mechanisms are related to H2S-induced changes in various signaling pathways as well as [Ca2+]i in the platelet. The interaction of H2S and platelets is also affected by lipid metabolism.
Keywords: calcium signaling, hydrogen sulfide, platelets
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