DOI: 10.5176/2345-783X_PHARMA14.09
Authors: Smita Thube, Satej Katekar, and Tasleem Kureshi
Abstract:
The present work deals with the comparative study of antivenom properties of Albizia lebbeck bark extract (ALBE) against the Cobra Naja naja venom with the standard marketed preparation. ALBE at a dose of 1280mg/kg was highly capable of neutralizing the lethality& decrease in the Creatinine Phosphokinase Activity levels induced by the venom and was capable of inhibiting phospholipase A2 dependent hemolysis of sheep RBCs in a dose dependent manner. With reference to the anticoagulant activity of Cobra Naja naja venom, with an increase in the concentration of the venom there was an anticoagulant effect which caused the prolongation in the clotting time. When tested for the bleeding time Cobra Naja naja showed more prolonged bleeding time from that of the normal mice, which indicates that the Cobra Naja naja venom contains the potent anti-coagulant property. ALBE was capable of neutralizing this anti-coagulation in the dose dependent manner. ALBE was found to be effective in neutralization of edema induced by venoms. There was a significant decrease in the edema (footpad thickness) at dose of 1280mg/kg of ALBE. Inflammation induced by the venom was significantly neutralized by the bark extract. The alcoholic bark extract significantly reduced the clinical symptoms associated with venom poisoning and also decreased the venom lethality. So, it is hereby concluded that the alcoholic extract of Albizia lebbeck bark can serve as a promising entity in Cobra Naja naja snake venom poisoning. The main chemical constituents present in the ALBE are tannins of condensed type, β-sitosterol, betulinic acid and its glycosides. lupeol, oleanolic acid, docosanoic acid, alkaloids, flavanoids [kaempferol and quercetin] which are the main proved anti-snake venom constitutents. Further the research is in progress to find out the active component responsible for the activity and to determine the possible mechanism of action at Haffkine Institute, Mumbai, India.
Keywords: Albizia lebbeck, Antivenom, Naja naja
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