DOI: 10.5176/2251-3140_2.2.34

Authors: Kirti Rani


Amylase is commonly known as starch hydrolyzing enzyme which degrades the starch into glucose monomers and limit dextrins by digesting it from the non-reducing end. Hence, this property of amylase makes it most industrial important enzyme with wide applications in food, pharmaceutical, paper, leather, detergent and textile industries. As well as,
enzyme immobilization is excellent way to bind the enzyme of interest onto stable carrier systems which was makes the immobilized enzyme more resistant towards pathogenic attack on storage as compared to free enzymes. Hence, immobilization increases the storage stability and reusability of bound enzyme for various continuous chemical and biological processes. In the present work, immobilization of Glycine max amylase was done onto variety of activated fabrics (silk, cotton and nylon) whose activation was made by treating them with sodium chloride and sodium nitrate.
Kinetic parameters (pH, incubation time, temperature, substrate concentration and CaCl2 concentration) were studied for Glycine max amylase enzyme by conventional dinitrosalicylic acid method. This amylase was found to be thermal stable and showed stability upon wide range of pH. A comparative study was done to know {6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} of retention of activity of Glycine max amylases on to the variety of chosen activated fabrics.

Keywords: Glycine max, silk, cotton, nylon, glutaraldehyde, sodium chloride, sodium nitrate

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