DOI: 10.5176/2339-5060_1.1.1
Authors: Audie Thompson, Danny Nguyen and Felecia Nave
Abstract:
Hydrogel membranes continue to be investigated for potential use in a number of applications including bioseparations, water purification, controlled drug release, to name a few. The potential of hydrogels to overcome disadvantages typically associated with packed bed chromatography such as low protein loading, elution conditions and regeneration increase their potential applicability and use for biological separations. However, these materials typically provide limited selectivity between proteins of similar size, low protein loading and durability. To overcome these limitations, poly vinyl alcohol (PVA) hydrogels were functionalized with metal affinity ligand to provide a selective means to enhance protein loading and improve protein separation characteristics. Additionally, the crosslink ratio was varied from 1.25 {6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} (v/v) to 5.0 {6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} (v/v) to further enhance membrane selectivity while also increasing membrane durability. Characterization and comparison of membranes with varied degrees of crosslinking and examination of structural properties and behaviors of membranes were determined for this study. Atomic Absorption Spectroscopy (AAS), Fourier Transform Infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM) were used to characterize and to examine IMAH membranes after functionalization. Results demonstrate successful functionalization of the poly vinyl alcohol membrane with metal affinity ligands. The FTIR results showed different band frequencies for specific groups associated with PVA cross-linked with glutaraldehyde. The presence of the chelator did not impact the swelling properties of the membrane; however, the Bound Copper Content (BCC) was increased by 50{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} for the IDA-Me2+ membranes as the crosslinking ratio increased from 1.25{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} to 5{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} GA (v/v). Moreover, an increase in cross-linking ratio resulted in structural changes that enhanced copper attachment which increased protein solubility.
Keywords: poly vinyl alcohol, Iminodiacetic acid glutaraldehyde
Updating...