DOI: 10.5176/2339-5060_1.2.9
Authors: Wei-Ting Chen, Chiung-I Wang, Shih-Feng Fu, and Yang-Wei Lin
Abstract:
A simple and inexpensive capillary zone electrophoresis (CZE) with UV detection method was used to determine salicylic acid (SA) and six structural analogs (benzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, acetylsalicylic acid, methyl salicylate, and jasmonic acid). All of the compounds were successfully separated within a migration period of 6 min, with a high number of theoretical plates (>37,000, calculated using the width at the base of the SA peak), in 20.0 mmol L−1 Na2HPO4/H3BO3 buffer (pH 9.0) containing 2.0{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} methanol and 2.0{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} acetonitrile. The regression line for SA was linear over the range 0.5–200 mol L−1. Both the intra- and inter-day precisions of the migration time (relative standard deviations, RSDs, 0.6{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} and 4.7{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}, respectively) and peak area (RSDs 0.9{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} and 7.3{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}, respectively) were acceptable. The proposed method was used to determine the SA concentrations in tobacco leaves (Nicotiana tabacum L.) from the Xanthi-nc (NN genotype) and the Nt-NahG mutant strains that had been irradiated with UV (254 nm) for 20 min and the ones that had not been irradiated. Rapid separation (<6 min), good reproducibilities (RSDs < 11.9{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}), and good recoveries (94.7{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} to 118{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) were obtained in the tobacco leaf analyses.
Keywords: Tobacco leaves; salicylic acid; capillary zone electrophoresis; UV detection
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