DOI: 10.5176/2251-3701_2.1.26
Authors: Felecia M. Nave, Audie Thompson and Veniece Kirksey
Abstract:
Previous studies have shown that functionalization of hydrogel membranes with metal affinity ligands increases the
separation potential of the membranes for purification of biological systems. To achieve separation, histidines that are exposed on the proteins surface are attracted and attach to the metal ion within the IMA membrane matrix. The effectiveness of these membranes depends on several factors including, but not limited to, type of chelating group, the metal ion, and solutionbuffer. This paper is concerned with crosslinking of poly (vinyl) alcohol (PVA) with varied degrees of crosslinking that was subsequently functionalized with the immobilized metal affinity (IMA) group, that included a spacing element, (1, 4 Butanediol Diglycidyl ether (1,4 BDE)), chelator (NTA (Nitrilotriacetic acid)), and metal (Nickel (Ni2+). The impact of chelating ligand and glutaraldehyde concentration on the swelling and bound
metal content was determined. Additionally, Fourier-transform infrared spectroscopy (FTIR) was used to examine the functional groups for the control (without metal attached) and affinity (with metal attached) membranes. Ultimately, this study suggests that crosslinking membranes with a higher glutaraldehyde percentage yielded a higher content of bound nickel.
Keywords: poly vinyl alcohol, Nitrilotriacetic acid, glutaraldehyde
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