Erythritolproduction from glycerol by Yarrowialipolyticain batch, fed-batch and repeated-batch regimes

DOI: 10.5176/2251-2489_BioTech13.62

Authors: Anita Rywińska, Ludwika Tomaszewska, Monika Bąk, Aleksandra Mirończuk, Krzysztof Cybulski, Waldemar Rymowicz

Abstract:

Erythritolisafour-carbon sugar alcohol,used as sucrose substitute. It is commercially produced only in biotechnological processes. The aim of this investigation was to compare yield and dynamics of erythritol production from glycerolbyY.lipolyticaWratislaviaK1-UV21 strain in batch, fed-batch, and repeated-batch processes. The cultivations were performed in Biostat BPlusbio reactor. The total concentration of glycerol was 150 and 250g/Linbatchand fed-batch culture, respectively. The repeated-batch culture was started as a fed-batch cultivation and next, after glycerol depletion, from the bioreactor 0.8L of the culture broth was taken and exchanged with the same volume of fresh medium containing 100g/L of glycerol. This procedure was repeated four times. In the batch and fed-batch cultures yeast produced 84.1 and 136.0g/Loferythritol. The production yield was similar in both processes, however the productivity and specific production rate was higher in batch culture. In repeated batch culture higher ythritolproductionyield(0.51–0.55g/L)anddynamicsofthebiosynthesis(0.8–1.05g/Lh) was observed during first three cycles.