Growth kinetics and carotenoid production of a newly isolated bacterium on glucose as a carbon source during batch fermentation

DOI: 10.5176/2251-2489_BioTech15.90

Authors: Ruchira Mitra, Amit Kumar Samanta, Surabhi Chaudhuri and Debjani Dutta

Abstract: A carotenoid producing bacterium was previously isolated during routine screening of pigment producing microorganisms and was identified as Kocuria marina. It was cultivated in a growth medium containing glucose, maltose, sodium chloride, bacteriological peptone and yeast extract and incubated at 25˚C at 150 rpm. The effect of varying initial glucose concentration (0 gL-1 to 20 gL-1) on bacterial growth and pigment production were studied in a batch mode. Monod model was used to describe the cell growth pattern. The values for μmax and KS were calculated to be 0.2499 h-1 and 0.4047 gL-1 respectively. The maximum pigment yield of 4.46 μg mL-1 was obtained at an initial glucose concentration of 7.5 gL-1 at 56h of incubation. The reducing sugar utilization was studied for all the experimental conditions.

Keywords: β-cryptoxanthin, Biomass, Monod model, Reducing sugar utilization