DOI: 10.5176/2251-2489_BioTech15.10
Authors: Sangeeta Choudhary, and Suman Kumari
Abstract: Bacterial strains isolated from textile industry waste site were characterized in terms of general metabolic profiles, antibiotic sensitivity and heavy metal resistance property. Initial screening of 20 isolates in presence of 0.25 mM (Cu, Cr and Zn) and 0.1 mM Cd revealed that 5 isolates (SNA1, SNA3, SNA5, SNA12 and SNA14) were multimetal resistant. These selected strains were further tested to determine minimum inhibitory concentration (MIC) for above metals. Most of bacterial strains could tolerate all the metals tested except SNA1 which was sensitive to Cd. Strain SNA3 was found most efficient in tolerating Cr (MIC 3.5 mM) in comparison to other strains. Overall we observed that the degrees of resistance to various metals differed among the isolates and SNA14 was the most tolerant strain in our collection, followed by SNA3, SNA5and SNA12. The ability of these strains to grow over a wide range of metal concentrations, along with antibiotic resistance, utilization of variety of carbon sources indicates metabolic diversity of the strains which clearly point to the potential application of these bacterial strains for metal remediation.
Keywords: heavy-metal; bioremediation; bacteria; metal-resistance
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