DOI: 10.5176/2251-2489_BioTech15.87
Authors: Kirti Rani and Chanchal Chauhan
Abstract: Nanoparticles have potential applications in industrial biotechnology, especially in biomedical, chemical, pharmaceutical and bio-electronic research fields. In our present work, bovine serum albumin was chosen as matrix for preparation of chemically modified amylase bound nanospheres which are biodegradable, biocompatible and non-toxic material. Amylase is known as industrially important enzyme for its applications in food, baking, brewing, fermentation, detergent, textile and paper industries. Cicer arietinum has rich source of amylase and its germinated seeds were used for extraction of amylase which was encapsulated into chemically modified bovine serum albumin nanopshere by desolvation method through covalent coupling by glutaraldehyde. {6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} of encapsulation of bound enzyme in prepared bovine serum albumin nanospheres was found 77.6{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465} and its characterization was done by performing Dynamic Light Spectroscopy (DLS) and Scanning Electron Microscope (SEM). The observed size of prepared amylase loaded bovine serum albumin by desolvation method was found to be in the range of 56nm to 107nm. Its storage stability was increased up to 12 months as compared to free enzyme which was found to be for one day only when stored at 4ºC. These prepared enzyme loaded nanosphere may be used as eco-friendly and cost effective bio-active vehicle for drug delivery and electronic metabolic robotic tool in therapeutics.
Keywords: Cicer arietinum amylase, Bovine serum albumin, Desolvation, Encapsulation, Nanoparticles
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