Production of Gallic acid using a Novel source Bark of Mimusops elengi

DOI: 10.5176/2251-2489_BioTech15.04

Authors: Dr.D.Sarvamangala, Deepthi.Sami, Dr.B.Sarada and A.V.Lalitha

Abstract: Gallic acid and its structurally related compounds are found widely distributed in fruits and plants. Esters of Gallic acid have a diverse range of industrial uses, as antioxidants in food, in cosmetics and in the pharmaceutical industry. In addition, Gallic acid is employed as a source material for inks, paints and color developers. Studies utilizing these compounds have found them to possess many potential therapeutic properties including anti-cancer and antimicrobial properties. The purpose of this study is to evaluate the production of Gallic acid using Mimusops elengi plant bark as substrate and to design a model to optimize the production using RSM. The micro organism used for this production is Aspergillus niger(NCIM No-1261). The production time for Gallic acid ranges from 10 to 15 days using this substrate. Maximum activity and production is obtained after 10 days with optimization of different parameter like pH, temperature and production time. The Gallic acid is extracted from the fermentation fluid using organic solvents and is analyzed using TLC, XRD and FTIR. The confirmation of Gallic acid is obtained by comparing with the standard.

Keywords: Gallic acid; Mimusops elengi; Response surface methodology; FTIR; XRD