DOI: 10.5176/2251-2489_BioTech13.25
Authors: Sucharita Sen, P.K. Roychoudhury
Abstract:
The monoclonal antibody 520C9 is specific for human breast oncoprotein HER-2/neu, that overexpresses in various metastatic breast and ovarian carcinomas. The production of monoclonal antibody 520C9 (mouse IgG1) from suspension hybridoma cell line HB-8696 is shown to improve by, using optimized perfusion culture strategy. The cells were grown under two different culture modes, namely, the batch culture mode and the perfusion culture mode, under constant inoculum cell density of 1.2 × 105 cells/ml. In perfusion culture, the optimum medium was introduced at different perfusion rates: 0.25, 0.5, and 0.75 vvd (defined as volume of fresh medium/working volume of reactor/day). The maximum viable cell density in both culture systems has been investigated while maintaining steady state conditions. It has been observed that monoclonal antibody productivity using perfusion strategy improved significantly than that observed in batch culture. The perfusion culture system is preferred over other traditional culture techniques such as batch and fed-batch system, since it provides higher cell density for prolonged periods, higher yield of product, besides less exposure to toxic metabolites and, less exposure of hydrodynamic shear stress to the cells. In this paper, we have shown that the perfusion culture system operates in a programmed manner, and can be easily applied to other culture systems.
Keywords: Monoclonal antibody (mAb) 520C9, perfusion culture, Hybridoma cell HB 8696, Oncoprotein c-erbB-2 and HER-2/ new gene
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