Production of mannitol by Yarrowia lipolytica in fed-batch fermentation

DOI: 10.5176/2251-2489_BioTech13.65

Authors: Piotr Juszczyk, Ludwika Tomaszewska, Waldemar Rymowicz

Abstract:

The effect of different concentrations of pure glycerol on yield and productivity of mannitol biosynthesis by Y. lipolytica S3 was examined in this study. All experiments were conducted in a stirred tank reactor Biostat B plus (Sartorius), on media containing 50250 g/L of glycerol.

The kinetic parameters, i.e. the yield, volumetric productivity and specific rate of mannitol production depended on the concentration of glycerol in the production media. Mannitol concentration varied from 9.7 to 54.7 g/L with production yield from 0.19 to 0.33 g/g and productivity from 0.22 to 0.58 g/Lh. In the batch culture S3 strain produced 54.7 g/L of mannitol when the production medium contained 175 g/L of glycerol. In addition, a large amount of erythritol was produced by yeast, especially in media with high concentration of the substrate.
Based on the results obtained in the first step two fed-batch cultures were performed with initial glycerol concentration of 50 and 75g/L. The culture broth was fed with three portions by pulsed additions to the total substrate concentration 200 and 300 g/L, respectively. With a medium containing 300 g/L of glycerol, the highest concentration of mannitol of was obtained and reached 85.6 g/L after 240 h. However, yeast accumulated a very large amount of erythritol (92.4 g/L). In the medium containing 200 g/L yeast produced 75.9 g/L of mannitol and 52.4 g/L of erythritol.