DOI: 10.5176/2251-2489_BioTech20
Authors: Pabbaraju Neeraja and K.Sujatha
Abstract: Ammonia is a normal constituent of all body fluids but can become a toxicant under high concentration. This leads to hyperammonemia. This state in human beings in quite fatal and is caused because of genetic defects in ornithine cycle enzymes. Selenium is a trace element which is toxic in large amounts but plays a vital role in the body being an integral component in certain enzymes, protecting cell membranes. Copper is an essential trace element that is vital to the health of all living things i.e. humans, plants, animals and micro organisms. The present study aims to examine whether selenium and copper alleviate ammonia stress conditions. The selected concentrations of ammonium sulphate, sodium selenite and copper sulphate after toxicity testing evaluation are 62.5mg/kg body weight for ammonium sulphate, 5.2mg/kg body weight for sodium selenite and 120mg/kg body weight for copper sulphate. These are administered through intraperitonial method to rats for one week. After one week treatment, detoxification enzymes like arginase and glutamine synthetase levels were estimated in liver and brain tissues of albino rats by using standard methods. The impact of sodium selenite and copper sulphate on these detoxifying enzymes of rat under ammonia stress would be discussed.
Keywords: ammonia stress, albino rat, detoxification enzymes, liver and brain
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