DOI: 10.5176/2345-783X_PHARMA13.20
Authors: Dr. Prakash R. Itankar, Sneha Phulambrikar, Prashant R. Verma and Sumit K. Arora
Abstract:
Ethnopharmacological relevance: Whole Plant of Tephrosia Purpurea is used as an antidiabetic drug in Ayurvedic traditional system of medicine.
Aim of the Study: To evaluate flavonoid rich fraction (TPSBF) Of Tephrosia purpurea stem for its antihyperglycemic and pancreatic regeneration potential in streptozotoc ininduced diabetic swiss albino mice.
Materials and methods: Induction of diabetes in swiss albino mice was carried out using streptozocin (180mg/kg; i.p). The ethanolic extract TPSEE and its broad fractions (TPSCF; cholorofoam and TPSBF; n butanol soluble fractions) were screened for acute and sub-chronic antidiabetic activity in dose range of 250 and 500mg/kg orally, along with that its antioxidant effect was also evaluated. Pancreatic regeneration was determined by histopathological and morphometric analysis. Quantitative and qualitative evaluation of extracr and its function was also done to ascertain the segregation of phytoconstituents.
Results: Most significant antidiabetic, antioxidant and pancreatic regeneration capacity was observed in TPSBF (flavonoid rich fraction).
Conclusion: It was concluded that fractionation of TPSEE has lead to obtain flavonoid rich fraction which was justified by the results of qualitative and quantitative estimation. Enrichment of polyphenols and flavonoids in TPSBF is also reflected by its significant free radical scavenging activity as compared to TPSEE. Enhanced antioxidant activity of TPSBF may have lead to reduce the oxidative stress generated due to diabetic state. Near to normoglycemic condition in TPSBF group is either due to the formation neoislets or replication of the existing ones. Thus, prominent antihyperglycemic and pancreatic regeneration effect of TPSBF can be attributed to its enriched polyphenolic and flavonoid content.
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