DOI: 10.5176/2345-783X_PHARMA13.25
Authors: Nalamolu Koteswara Rao, Krupavaram Bethala and Sreenivas Patro Sisinthy
Abstract:
The objective of the research study is to evaluate effect of Orhtosiphon stamineus in streptozotocin induced diabetic rats. Orhtosiphon stamineus roots were first extracted with petroleum ether and the remaining residue was extracted with methanol. Various doses of methanolic extract (300 mg/kg and 600 mg/kg) were administered for three weeks to streptozotocin (STZ) induced diabetic rats. Changes in blood glucose levels were monitored at weekly intervals. At the end of the experiment, changes in hepatic Glucose-6-phosphatase, Glucose-6-phosphate dehydrogenase, glycogen, glucokinase and hexokinase were evaluated. Glibenclamide (600 μg/kg) was used as standard drug for comparison. Significant reduction in blood glucose levels (p<0.01) was observed with both the doses and dose dependent effect was observed. O. stamineus has shown significant reduction in glucose-6-phosphatase and significant increase in glucokinase, hexokinase, glucose-6-phosphate dehydrogenase, glycogen levels. Results showed that O. stamineus possessed significant antihyperglycemic activity in STZ induced diabetic rats which is due to attenuation of glucokinase, hexokinase, glucose-6-phosphate dehydrogenase, glycogen storage and reduced glucose-6-phosphatase which rationalizes its promising use for treatment of diabetes in future.
Keywords: Orhtosiphon stamineus roots, Streptozotocin induced diabetes, rats, blood glucose levels, glucokinase, hexokinase, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, glycogen
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