DOI: 10.5176/2251-189X_SEES19.71
Authors: LK. P. Lisha and Debasis Sarkar
Abstract: A dynamic flux balance analysis of bioethanol production potential of Zymomonas mobilis from glucose/ xylose mixtures is presented. Z. mobilis is glucose-selective microorganism and two types of community-culture schemes are investigated for effective utilization of both glucose and xylose: co-culture and sequential co-culture. For glucose/xylose mixture, the co-culture fermentation of 3:1 glucose/xylose mixture using substrate-selective microorganisms (wild-type Z. mobilis and xylose-selective recombinant Escherichia coli strain ZSC113) predicts an ethanol yield of 0.43 g/g and this can be further improved to 0.49 g/g by deletion of suitable genes from ZSC113. The sequential co-culture is considered for substrates having separated glucose and xylose fractions using two wild-type microorganisms, Z. mobilis and Scheffersomyces stipitis and this predicts an ethanol yield of 0.48 g/g from substrate having glucose and xylose in 3:1 ratio.
Keywords: Dynamic flux balance modelling, Batch fermentation, Bioethanol, Community-culture, Z. mobilis
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