DOI: 10.5176/2251-189X_SEES19.66
Authors: Musa M. Musa
Abstract: The ability to control enantioselectivity of enzymes through protein engineering is an expensive tool, thus other less tedious approaches are of interest. Here, we report the effect of concentration of 2-propanol co-substrate, which is also used as a co-solvent, on the enantioselectivity of a secondary alcohol dehydrogenase from Thermoanaerobacter pseudoethanolicus (TeSADH) mutant. More specifically, we used W110A TeSADHcatalyzed asymmetric reductions of 1-phenyl-2-propanol and 1phenyl-2-butanol to study the effect of changing the reaction medium on the enantioselectivity. The ability to control the stereoselectivity of TeSADH by changing the concentration of 2propanol and other co-solvents is an important tool that can be used in deracemization of alcohols to obtain their enantiopure forms in high efficiencies.
Keywords: Alcohol dehydrogenases, co-substrate, enantioselectivity, redox reactions
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