DOI: 10.5176/2382-5685_VETSCI13.55
Authors: Christopher B Riley, Siyuan Hou, Monchanok Vijarnsorn and R Anthony Shaw
Abstract:
The focus of osteoarthritis (OA) research has shifted from microscopic changes in cell number and total protein parameters, to the search for biomarkers (direct or indirect molecular indicators of abnormal skeletal turnover) for joint disease in horses. Infrared (IR) spectroscopy has been established as a useful tool for the assessment of biological molecules and the biochemical response to disease. Synovial fluid was collected from 105 normal clinically normal antebrachiocarpal (AC), midcarpal (MC) and tarsocrural (TC) joints. Thin films were prepared and infrared absorbance spectra in the mi-infrared (MIR) region of 400-4000 cm-1 recorded using a FTIR spectrometer equipped with a deuterium tryglycine sulphate detector. For each acquisition, 512 interferograms were signal averaged and Fourier transformed to generate a spectrum with nominal resolution of 4 cm-1. Spectra were preprocessed and left and right spectra compared by ANOVA. Between joint comparisons were made using a novel modified method stemming from of partial least squares discriminant analysis and projection pursuit methods that maximized the difference of between-group variance minus within-group variance. Differences within animal between left and right joints were minimal. Differences among AC, MC and TC synovial fluid spectra were significant. The finding of a broad range biomolecular differences among these joints supports indicates that interarticular variation within the horse needs to be considered.
Keywords: infrared spectroscopy, osteoarthritis, synovial fluid, discriminant analysis
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