Isolation, Purification and Characterization of Immunoprotective Effects of H11 and H-Gal-GP from Indian Isolate of Haemonchus Contortus

DOI: 10.5176/2382-5685_VETSCI13.79

Authors: Muthusamy Raman, Manikkavasagan Ilangopathy and Azahahianambi Palavesam

Abstract:

Immunoprotective microsomal aminopeptidase (H11) and H-Gal-GP were purified from the gut membranes of an Indian isolate of Haemonchus contortus by peanut and Concanavalin-A lectin affinity chromatography. SDS-PAGE profiles of the elutes of Con A and Peanut lectins revealed bands at 110 kDa of H11 and the four bands, ~ 250, 170, 45 and 35 kDa of H-Gal-GP respectively. Eighteen Mecheri lambs were divided into three groups of six each. Group I was immunized with three doses of 500 μg of Con A H11 fractions on day 0, 28 and 49, Group II with 500 μg of H-Gal-GP and Group III was treated as adjuvant control. Group I, displayed a reduced faecal egg count (85.1{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) and worm burden (77.5{6e6090cdd558c53a8bc18225ef4499fead9160abd3419ad4f137e902b483c465}) following larval challenge on day 70 post immunization. The humoral immune response rose significantly from day 42 and maintained till day 132 post immunization. During the same period, the lympho-proliferative response showed a higher stimulation index in immunized lambs (1.5102) than unimmunized controls (0.9604). The haematological values remained within normal range in immunized animals, whereas a marked reduction in both PCV and Haemoglobin levels were observed in unimmunized control animals following the larval challenge.

Keywords: Haemonchus contortus, microsomal aminopeptidase, H11, H-Gal-GP immunization, Sheep